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They also perform some key posttranslational modifications not possible in bacterial systems.

Protein extraction was performed following some modifications of the procedure of Muhamad et al. (2012).

PCR-RFLP for DNA extracted from FF-PET was performed with some modifications: forward primer was replaced by 5'-TAA CTT GAA CCA TCT TTT AAC the3', the annealing temperature was reduced to 61°C, electrophoresis was performed in 3% agarose gel for 70 min (10 min longer).

Mitochondrial purification was performed with some modifications as described previously.

A histidine-dependent growth assay of H. influenzae isolates was performed with some modifications [ 24].

To detect new loci, a subtractive hybridization approach [ 52] was performed with some modifications.

SDS-FRL was performed with some modifications to the original method (Fujimoto 1995).

The assay published by Porsch-Ozcürümez et al. [ 11] was performed with some modifications.

For sera from animals immunized with H7N9-based vaccines, VN was performed with some modifications from the H1N1 protocol.

(49) Embedding the cerebral ganglia in sucrose was performed with some modifications as described by Kabotyanski et al. (50) Briefly, the ganglia were transferred onto a glass coverslip where ASW was substituted by a sucrose solution (1 M sucrose, 10 mM CaCl2).

The FRAP (Ferric Reducing Antioxidant Power) assay developed by Benzie and Strain [ 33] was performed with some modification.

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