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Given the dimensions and uniqueness of the samples from the paintings, it was not possible to perform replicate analysis on different sample aliquots, and thus it was not possible to perform the t test.
Due to the limiting amount of sample available we were unable to perform replicate PCR runs, but each qPCR experiment was itself ran in triplicate.
Therefore, it is not necessary to perform replicate microarrays on specimens, replicate clones on each microarray, or provide data on intra- and inter-assay variability measurements; all the data required are the 20 pairs of data points for each gene available on the website.
Of these, due to limitations on the available plasma volumes and our desire to perform replicate analyses, four to six rearrangements per tumor were selected (again to reflect a variety of copy number states) and the corresponding probe was synthesized for droplet digital PCR (ddPCR) analysis of patient plasma samples.
Similar(56)
We performed replicate SOX2 ChIP and IgG ChIP.
Thus, we performed replicate sequencing runs for each of the PGM and MiSeq libraries.
We have performed replicates for all expression profile analyses and obtained consistent expression patterns between replicates.
Specifically, we perform k replicates.
We did not perform technical replicates on DNA methylation analyses.
All experiments were performed in replicate independently.
Experiments were performed in replicate.
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