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As the name implies, the technique uses chromatography to separate the substance under study into its components, and spectrometry to perform mass analysis of the components.
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The performance of a miniature, stainless steel, rectilinear ion trap (RIT) with dimensions of 1.66 mm × 1.43 mm (x0 and y0 respectively) is characterized by sampling externally generated ions and performing mass analysis without benefit of differential pumping to simulate conditions in a miniature system.
In this work, a Varian (Walnut Creek, CA, USA) 3600 GC (gas chromatography) system coupled with a Saturn quadrupole ion trap mass spectrometer was used to perform mass spectral analysis in real-time catalytic cracking reactions.
To identify the amino acid residue phosphorylated by Akt and PKC, we decided to perform mass spectrometric analysis from recombinant Dnmt1 protein (Dnmt1R) phosphorylated by Akt and PKC.
Future experimental work could specifically isolate the vacuoles of the NC through centrifugation methods and then perform mass spectrometry analysis on these fractions, which might reveal more focused spectrum searches.
KAO conducted proteomic experiments, performed mass spectrometry analysis and conducted bioinformatic analysis of the data.
We thank Berthold Gaßen for antibody generation, Debora Hofmann and Philipp Niehues for help with immunofluorescence analysis, and Dr. S. Müller (CMMC) for performing mass spectrometry analysis.
We performed mass spectrometry analysis of immunoprecipitated LYL1-V5 digested with endoproteinase Lys-C and trypsin.
We isolated only the proteins unique to the transgenic (compared to non-transgenic control) and performed mass spectrometry analysis.
We have performed mass spectrometric analysis of the purified proteins and the results confirmed that the proteins are indeed OR131-2 (Supplementary Figure S3).
We thank Beryl Ortwerth for helpful discussions and critical reading of the manuscript and Beverly DaGue for performing mass spectrometry analysis.
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