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Since measurement of fluorescence is easy to perform, labelling of PMN with a fluorescent marker has been proven to be a very feasible option.
In vitro transcription was then achieved in order to amplify the initial transcripts quantity, concomitantly with aminoallyl-dUTP incorporation to perform labelling with cyanins (Cy5 for the reference and Cy3 for samples).
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For each TF unit within a nonoverlapped T-segment, we perform labeling as follows: (7).
To further understand the effects on glucose, we performed labelling experiments with [1-13C] [1-13C] to quantify the metabolic fluxes in the central carbon metabolism.
We performed labeling and hybridization according to the manufacturer's protocol.
Finally, we performed labeling experiments with a 24 h Ac4ManNAz incubation between the first and second DIFO reactions.
We performed labeling experiments using antibodies to CD31 (endothelial cell marker; green) and fibroblast markers FSP1 (red, also termed S100A4).
This platform can perform label-free and rapid single-cell capture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biological species, sorting, concentration, positioning, enumeration, and characterization.
Human tracking across non-overlapping cameras establishes detected/tracked human objects' correspondence between two non-overlapping cameras so as to successfully perform label handoff.
M.L., X.M. and D.S.T. performed labelling and smFRET imaging.
However, MST can also be performed label-free by exploiting intrinsic protein UV-fluorescence.
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