Also, the second contribution includes the specification on how to deal with and preserve shared instances when performing import or export using the flyweight design pattern.
The authors thank Sabine Freitag and Pia Meyer for their perfect technical assistance and Uwe Langner and Ralf-Bernd Klösgen for performing import experiments of PpAOS1 + 2 in pea chloroplasts (Martin-Luther-University, Halle/Saale, Germany).
For this purpose, we radiolabeled the COX6a subunit of complex IV and the UQCRFS1 subunit of complex III and performed import experiments in freshly isolated heart and liver mitochondria.
In one step, select the photos you wish to delete after import, perform the import, then choose "Delete" when done.
Choose an option to handle duplicates while importing, and click Choose an option to handle duplicates while importing, and click to perform the import.
PP performed mitochondrial import studies as well as DNA binding studies and the respective data analysis.
To corroborate the immunofluorescence findings, we performed mitochondrial import assay of radiolabeled in vitro-translated Cterm and MTS-Cterm.
As a control for the biological relevance of the assay, we performed several import assays on ice at 0°C.
AtGTR1 likely performs a role in distributing glucosinolates within the leaf, possibly performing an import function into glucosinolate-rich cells adjacent to the phloem [ 14].
All statistics were performed by importing the data into R v.3.1.2 [ 38].
Morphometric analysis was performed by importing Neurolucida tracings into NeuroExplorer software version 3.70.2 (MicroBrightField, Inc) [ 21].
