Mr Azman still argues against selling poorly performing hi-tech firms, such as SilTerra, lest that deprive Malaysia of the associated industry and technology transfers and the jobs that come with them.
To further understand these changes in nuclear architecture in growing and senescent cells, we performed Hi-C, a proximity-based ligation assay that measures the frequency of close chromatin interactions, as summarized in Figure S1C (Lieberman-Aiden et al., 2009).
As we have now performed HI-MS analysis of Ng peptides in more than 75 plasma samples in total and the plasma-specific peptides have been present in all of these, we can conclude that these are consistently found in plasma and are thus most likely not caused by the preanalytical handling, In a previous study, we showed increased CSF Ng levels in AD using ELISA [ 16].
To ensure infection, we obtained sera samples at 13 dpi and performed HI assays using chicken red blood cells.
Serum samples were screened for Newcastle disease virus (NDV specific antibodies by performing HI assays with inactivated reference antigen and positive- and negative-control serum.
To monitor the antigenic similarity among the H9N2 viruses, we generated post-infection ferret antisera and performed HI assays using chicken red blood cells.
Previous studies have demonstrated that performing HI tests using horse RBCs could increase the detection of HI antibodies against avian influenza viruses in human sera [ 13, 14, 17- 19].
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2334/14/369/prepub We thank Liao YH for performing HI assay, Chang WT for assisting SAS and Lydia Wang for English editing.
A high-throughput system that performs HI assays in parallel with flow cytometer based assays, for example, may offer the best means to test and validate improved influenza serotyping methods.
We assessed the antigenic relationship of A/common buzzard/Bulgaria/38WB/2010 with other influenza (H5N1) viruses by performing HI assays with a panel of postinfection ferret antiserum against viruses from clades 2.3.2, 2.3.2.1, and 2.3.4; the panel was produced at St . Jude Childrens Research Hospital (Memphis, TN, USA; Table).
In order to obtain information about sensitivity and specificity of the easy-to-perform HI test, each serum was submitted to two different assays: an HI test and an USUV-specific PRNT-90, the "gold standard" in flavivirus serology.
