We performed further experimental verification and characterization of selected TIC and fusion events using quantitative RT-PCR and comparative genomic hybridization microarrays.
Finally, a parametric analysis is performed for further verification of the developed GEP models.
Sensitivity and parametric analyses are performed for further verification of the model.
The second experiment was performed for further verification of the findings of the first experiment, with experimental settings that simulate the T1D patients we imaged with 111In-exendin.
Specific PCR using 16S-rDNA and the lytA gene was performed for further verification of the presence of S. pneumoniae (data not shown).
In some somatic whole-cell recording and all dendritic recording experiments, neurons were filled with biocytin (0.2 1 %, Sigma, St . Louis MO) during recording and morphological reconstruction was subsequently performed for further verification.
In addition, further verification is performed against many field recordings of wheel squeal on a sharp curve of 300 m.
For further verification, we performed a time course followed with quantitative RT-PCR on the majority of transcripts with unknown associations to LPA, excluding CXCL1, CYR61, EGR1, IL-8, FN1 and PLAU.
Further verification was performed using IL-8 gene expression analysed using SYBR® Green based qPCR (Invitrogen), using GAPDH as a housekeeping gene.
For further verification, we performed hybridizations of H3K4me3 ChIPs from samples V425, V432, V441, SW480 and two independent preparations of normal colon mucosa to NimbleGen 385K promoter arrays and repeated the data analysis.
