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The QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) was used to perform extension reactions with overlapping primers, designed within the corresponding HCDR3 regions.
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Two complementary approaches were performed: extension of the sequences by PCR walking and PCR amplification of larger internal sequences using specific primers (see Methods and Additional file 4).
"Mount attempt": the male attempted to mount the female, generally from her side or back, performing extension and retraction movements of the tongue.
Knee extension (1st-4th week): The patient was in a chair at 90° of knee flexion and the hip performed extension starting from 90° and finishing at 45° of knee flexion.
The idea is always to perform an extension and jump over a mismatch to perform another extension until the number of allowed mismatches are attained.
If extension of one strand fails to close a given gap, GapCloser will perform another extension on the complementary strand.
The experiments perform an extension followed by compression.
We first execute our method for spatial synthesis and the enlargement of the frame size; after that, we perform the extension in the temporal domain.
Subjects were then randomized to ride a stationary bicycle, perform lumbar extension exercise, or receive spinal manipulation.
Subjects were asked to perform knee extensions and plantar flexions as fast as possible without a countermovement.
22 The following exercises were performed: back extension exercise, abdominal curl exercise, seated row, leg curl, leg extension, leg press, latissimus pulldown, and standing calf raise.
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