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The 8 DVD deficient and 8 control sample tissues were used to perform duplicate 2DE analyses, providing a total of 16 gels for each group.
We did not perform duplicate measurements which eventually could have improved the concordance.
RNA from the shRNAi clone, mock, and TC71wt from the early and late stages was isolated and used to perform duplicate hybridisation experiments with the Affymetrix HG-U133A platform.
To check the intra-examiner reliability, in accordance with the recommendation of World Health Organization (WHO) that each examiner should perform duplicate examinations on 5-10% of the sample [ 24], 6.23% of the sample was re-examined at the same visit, and Cohen's kappa coefficient and interclass correlation coefficient (ICC) were analyzed [ 25, 26].
Similar(56)
Instead, we performed duplicate experiments for selected conditions.
ELISA tests were performed duplicate in all samples.
All experiments were performed in duplicate.
All qPCR reactions were performed in duplicate.
All amplifications were performed in duplicate.
The entire procedure was performed in duplicate.
Three independent experiments were performed in duplicate.
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