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These platforms explored the detection of ultra-low concentrations of target biomarkers and have realized rapid, ultrasensitive and cost-effective bioassays requiring minimum sample volumes, which will enable primary care physicians and patients to perform assays in their respective settings, using so-called point-of-care diagnostics.
For both procedures, PP2A-A α knockdown PC-3 were trypsinised and used to perform assays.
LNCaP cells treated with 4 μM sorafenib underwent massive apoptosis resulting in an insufficient number of cells to perform assays.
This point is critical both in view of the time required to perform assays with vertebrates and the need of ethical adherence to the 3R principal in the use of experimental animals.
Therefore, samples of Austrian wine and grape juice were prepared to perform assays with the aim of evaluating these enzymes' performance on different natural substrates under varying conditions (pH, sugar content) and in comparison to fungal glycosidases.
The researchers perform assays that measure the effect of mutations in the genome.
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Synovial fluid samples were stored at −80°C prior to performing assays.
Overall, the three antigen assays performed similarly well.
Permeability assays were performed at 23 °C, except for activation energy (Ea) assays where temperature ranged from 10 to 34 °C.
Genotyping assays were performed by each group separately [ 1] (see Table 1 for assay description).
Susceptibility assays were performed at least two independent times.
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