Although the database is reliable, we performed additional validation of the data for the stocked lakes used in this study for further improvements.
To perform additional tests for model validation, the delivered power, (P=V^{2}/R_{mathrm{e}}), and the predicted power, (hat{P}=hat{V}^{2}(bar{theta })/R_{mathrm{e}}), versus the current I, at different steady states, both expressed as density, are compared and depicted in Fig. 7.
However, to ensure that the treatment of replicate cell line samples as independent samples in our model did not result in cross-validation bias, we performed additional internal validation experiments.
Out of these 600 INDELs, 97 were covered with more than 1,000 reads in the previous MiSeq data set reported by Narzisi et al. Hence, we only performed additional Miseq validation on the remaining 503 loci [ 40].
To assess whether our imputation strategy would yield similar benefits in African populations outside the HapMap 3 set, we performed additional cross-validations in a Gambian dataset from the Malaria Genomic Epidemiology Network (MalariaGEN; Malaria Genomic Epidemiology Network 2008).
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We were not able to perform such additional validation tests and, therefore, careful interpretation of the data is required.
In order to expand the experimental validation of candidate COUP-TFI target genes and to strengthen the 'proof-of-concept' of our methodology we performed additional in vitro validation experiments using wild-type and COUP-TFI−/− MEFs.
MethylC-seq and BS-PCR validation results of ENSGALG00000006522 (HCK) were performed (Additional file 12), and differentially methylated region was highlighted by light gray (Additional file 12).
Further, to reduce any biases we performed two additional validations in additional data sets.
No study performed any form of additional validation.
