Before using this method, we performed a validation experiment comparing the standard curve of the reference and the target to demonstrate that efficiencies were approximately equal [ 29].
In order to estimate the technical quality of the micro array data we performed a validation experiment using quantitative RT-PCR (qRT-PCR).
We then performed a validation experiment in 6-well format by withdrawing DOX and culturing the cells in 2i/L supplemented with each hit compound for up to 10 passages.
Thus, we performed a validation experiment and we plotted the log input amount of cDNA (dilutions of 1 20, 1 200, 1 2000, 1 20000 of a back fat tissue cDNA sample) versus de ΔCt, obtaining an absolute slope of 0.0273 (<0.1) thus permitting the use of the 2-ΔΔCT method for relative quantification.
Finally, to estimate the parameters of the meta-models, we further split each testing fold from within-cohort prediction in 10 sub-folds and perform a cross-validation experiment to train the meta-model and assess its performance.
In conclusion, by performing a simple validation experiment, real-time PCR assays based on human sequences, which are easily available, can be applied for analysis of baboon samples.
Fourthly, a validation experiment is performed.
A validation experiment was performed in a standing wave tube to measure the power dissipated and transmitted by a nonlocally reacting liner under moderate and high SPLs.
To verify the feasibility of this crack sensing principle, a validation experiment was performed using three groups of fiber winding shafts with different diameters and three groups of working light sources with different wavelengths.
For each of the genes, a validation experiment was performed.
After 14 months, a validation experiment was performed using an independent lot of BCR/ABL1 Quant reagents.
