Sentence examples for perform a buffer from inspiring English sources

In order to prepare plant-produced HA for animal trials, ground plant extracts were clarified and diafiltered to enrich and concentrate HA and to perform a buffer exchange.

When a sample containing one or more possible ligands to the target protein is injected into the system, the ligand(s) will bind to the immobilized target protein, thus facilitating sample cleanup, preconcentration, or performing a buffer change before detection.

First we performed a primary buffer screening where we tested thermal stability of G-CSF in different buffers, pH values and buffer concentrations.

They also perform a numerical study to determine buffer capacity.

A titration was performed with a buffer alone to account for the change in fluorescence due to dilution, which was subsequently subtracted from the titration performed with the ligand.

The enzymatic degradation was performed in a buffer solution with Rhizopus delemar lipase at 37°C.

The advantage of this approach is to allow the analyses to be performed over a buffer centered on the current axis instead of using a wider region.

ITC experiments were performed in a buffer composed of 20 mmol/L Tris (pH 8.0) and 150 mmol/L NaCl at 25°C using an iTC200 calorimeter (GE Healthcare).

Subsequent overnight hybridization was performed in a buffer with 0.5 µg/ml riboprobes at 65°C.

Measurements of iNOS activity were performed in a buffer containing 5 mM EGTA as a calcium chelator.

The purification was performed in a buffer containing 0.1% (w/v) DDM, and in the presence of 100 µM TCEP in order to stabilize the protein.