Yet, methylated DNA is perfectly well transcribed in Xenopus oocytes until it becomes chromatinized, recruits methyl-DNA-binding protein and HDACs (Jones et al, 1998).
Presumably, an siRNA is formed by two perfectly complementary RNAs transcribed by two reversely positioned promoters from one DNA template; these complementary RNAs are then hybridized and further processed into 20 25-bp 20 25-bp by RNaseIII enduplexescleases, namely Dicer.
But there has been a switch: their words have been transcribed and then perfectly lip-synched by the person being discussed, not by the original speaker.
However, given the filtering method used herein, such contaminating sequences would have to map perfectly to the Pst genome, be transcribed exclusively during infection, and have never been observed in previous wheat transcriptome or miRNA studies.
Also, the general acting, as keyed by Walter Slezak's hefty capering as the scientist, can best be described as roguish.But the picture, along with the camera, does flow along, the sets are ornate and aptly expansive, the color is perfectly beautiful and the Delibes music, stunningly transcribed on the sound track, never sounded better.
"You know, like Bugs Bunny is running down the street and a bowling ball hits him and there's a hole in him and through it you can see a tree and somebody's flipping an egg all that cartoon stuff but what he does is transcribe it perfectly.
The duo took turns "interviewing" one another, pretending to be Hughes as they transcribed imaginary conversations in which the movie producer described his friendship with Ernest Hemingway (fake) and his efforts to perfectly capture the breasts of actress Jane Russell on film (real).
This perfectly illustrates the biological dependence between microRNA and its host genes (i.e. the pri-miRNA transcript must be transcribed along with the mRNA to be processed and then detected on the microarray as mature miRNA).
Then the researchers transcribed the pieces, handed out the sheet music, had the pianists practice until they could replicate their colleagues' improv perfectly, and recorded their performances.
