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This process is continued until all the nodes are perfectly localized into the network.
Moreover, the subcarrier filters, though perfectly localized in time, spread out in the frequency domain, resulting in spectral leakage.
Even with perfectly localized dye molecules the duty cycle will influence the resolution of structures [ 19].
It indicates that different phases of ischemic stroke could be distinguished accurately when lesions have been perfectly localized and distance has been properly defined.
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Interestingly, iMELK dots perfectly co-localize with ZO-1 (open arrowheads in orthogonal projections) indicating that iMELK is concentrated at the tight junction.
This type of spatial organization perfectly explains the fact that in the chicken β-globin gene domain, the area demonstrating the cell-lineage-specific sensitivity to DNase I perfectly co-localizes with the genomic segment flanked by insulators.
This may explain the fact that in chickens, the borders of erythroid cell-specific domain with preferential sensitivity to DNase I perfectly co-localize with the borders of β-globin gene domain marked by insulators.
Using the αe1 antibody, we confirmed the absence of labeling on KO tissues while the staining of WT samples revealed the expected nuclear punctuated labeling which co-localized perfectly with the DAPI signals.
Furthermore, confocal images also revealed that not only the yeast and human H2B proteins were co-localized perfectly together in NIH-3T3 cells, their nuclear distributions interestingly contrast DAPI staining (see additional file 1: Figure S6), which is more selective for transcription inactive regions of the heterochromatins [ 29].
However, RPGRIP1α1 co-expression with RPGR1 19 did not alter the localization of RPGR1 19 in the ER network, but it caused the targeting and retention of RPGRIP1α1 to this network, where it always co-localized perfectly with RPGR1 19 in all co-transfected cells (Fig. 3D, upper panel).
GCS90 is exclusively located in the ER and perfectly co-localizes with the ER marker mRFP-HDEL, but not with the late Golgi marker ST-mRFP.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com