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Acidic monomers also increases the hydrophilicity of the material [24], which can increase the percentage of solvent retained in ethanol-based mixtures even after air-drying evaporation [25].
Initial solvent conditions were maintained for 6.3 minutes, at which time, the percentage of solvent B was increased linearly to 85% by 6.4 min. This solvent mixture was maintained until 9.5 min when the percentage of solvent B was increased to 100% to wash the column.
As the percentage of solvent accessible R, K, P, and T sites within BSA is close to 80%, our results suggest no over-representation of solvent accessible CS within carbonylatable sites.
Each new gradient was defined by a function giving the percentage of solvent B at every minute during the chromatographic run.
As an example, Figure 2C illustrates the nonlinear gradients designed to uniformize the distributions in Figure 2A and B, with each gradient described as a function giving the percentage of solvent B at every minute of the gradient run.
The percentage of solvent B to the total flow was changed to 5%, 45 %, 80 % and 5at 0 min, 10 min, 80 min, and 100 min point, respectively.
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The percentage of solvent-exposed negatively charged residues (Asp and Glu) is higher for alkaline-stable β-mannanases than for neutrophilic and acid-stable β-mannanases, which results in a higher ratio of negatively to positively charged residues.
The reverse-phase chromatography was performed on an Agilent High Capacity Chip (143 mm) using solvent A as the initial mobile phase and varying percentages of solvent B (90% acetonitrile, 0.1% formic acid) to constitute a gradient elution.
The percentages of solvent-accessible surfaces were rendered as a function of probe radii.
The linker-drug in T-DM1, SMCC-DM1 is hydrophobic and requires certain percentage of organic solvent such as DMA in the conjugation solution, limiting the manufacturing process in an organic-solvent-compatible device and adding extra costs.
After a screening of six different polysaccharide based chiral stationary phases and four co-solvents, the percentage of co-solvent, the flow-rate and the outlet pressure were optimized through a design of experiments (DoE).
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