The figure shows the percentage of reads from N2 worms mapped to various genomic regions Table 1 Genomic distribution of mapped reads in all samples.
The percentage of reads that aligned to the target region was more consistent between the SureSelect libraries.
A small percentage of reads (0.02% to 0.05%) were removed from the analysis prior to mapping to the reference, due to low quality.
fFiltering on percentage of reads made from mate-pair rescue.
NextClip will report the percentage of reads in each category and the percentage of reads exceeding the minimum length.
As the percentage of reads assembled decreases, so does the contig length, the number of chimeras and the percentage of reads matching their original genome, whereas the percentage of reads within a viral-bacterial hit increases.
Left panel: percentage of reads mapped to genome, and the percentages of reads that are unique ('percent_unique') and mapping to rRNA ('percent_ribo') out of those mapped.
It had the lowest percentage of reads assembled, the lowest N50 value and the highest percentage of reads within a viral-bacterial hit (Tables 1 and 2).
Right panel: percentage of reads mapping to exons ('percent_exons'), and out of those the percentage of reads in CDS regions ('percent_cds'), 3′ UTRs ('percent_3p_utr'), 5′ UTRs ('percent_5p_utr').
The one labelled 'R' shows the percentage of reads that have been correctly aligned, and the column labelled 'W' shows the percentage of reads that have been wrongly aligned.
The genome proportion of each cluster was calculated as the percentage of reads.
