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When we quantified by qPCR amplification of a 73 bp fragment the DNA that had been irradiated for 10 minutes in the UV crosslinker at 1 cm, 6 cm and 12 cm from the light bulbs, the percentage of amplification prevention achieved was 99.7±0.3%, 96±0.6%, and 89±1.9%, respectively (Figure 2).
Careful design of microsatellite primers is important in obtaining a high percentage of amplification.
As expected, the highest percentage of amplification was inside Bactrocera, with a mean of 49.1%.
The RiboPure™ kit samples showed the highest percentage of amplification and hence the largest amount of DNA contamination.
Percentage transferability was recorded as percentage of amplification of the SSR markers amplified in different accessions tested.
Universality: we estimated the percentage of amplification success on the first trial as an indicator of universality, using the same PCR conditions.
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If the 5p arm and 3p arm miRNAs have different affinities with adapters, they may have unequal percentages of amplification, causing biased estimates of expression levels and false reversal tissue preference.
To determine the sensitivity, we performed a 2-fold dilution of pBLV-IFconc and multifold dilutions of pBLV-LTRconc to give a range of provirus copy numbers from 100 to 0.78125, and examined after triplicate PCR amplifications the percentage of successful amplification.
For 2+/3+ discrepancies FISH test should always be added because the percentage of HER2 amplification is high.
It is and would be difficult to obtain a subset of TOP2A-amplified patients for the review of response to neoadjuvant therapy as neoadjuvant patients are more likely to be triple negative with a lower percentage of TOP2A amplification present.
For time points 0 34 days, we extracted DNA from approximately 0.30 × g of soil and found that a 1 20 dilution of DNA resulted in the highest percentage of successful amplifications.
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