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Each result and error bar graphed represents the percentage input of the mean ChIP signal in each region and standard deviation (s.d). calculated from three biological replicates.
Migration is presented as percentage input cells.
The final value was the percentage input obtained with specific antibody minus the percentage input obtained with anti-FLAG control antibody.
with results expressed as percentage input signal after normalizing Ct values from each primer set.
The percentage input was calculated as follows: 100 × (cells migrated to chemokine/total cell number).
The qPCR data were analysed and presented using the Percentage Input {100×2[Input CT −IP CT)]} method.
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Percentage inputs were calculated by the formula: % input = 100 × (2 x (PCR efficiency ) ] ^ [ − deltaCp (ChIP − Input ) ) × dilution factor (ChIP ) / dilution factor (input ) × (% of extract used as input ). A file containing the sequences of the primers used is available (see Additional file 6).
Data were analyzed with the percent input method: percentage of input was calculated by 100 × 2 CtInput−CtEnriched).
All PCRs were performed in triplicate and threshold amplication cycle numbers (Tc) using iCycler software were used to calculate IP DNA quantities as percentages of corresponding inputs using the following equation: IP DNA as a percentage of input = 2 ΔTc) × 100, ΔTc = input DNA Tc − IP DNA Tc.
ChIP qPCR data were expressed as either a percentage of input (y axis labeled "% Input") or enrichment relative to a control polycomb target site (y axis labeled "rel. enrichment").
Percentage of input was calculated as 100×2[Ct input) – Ct IP ], after adjusting the mean input Ct value for 1/5 starting material (fraction of input chromatin reserved).
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