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The percentage control growth (100%×[Aexp Aini]/[Afin Aini]) was expressed as a percentage of untreated controls.
Percentage control of variability was calculated using a coefficient of determination (r) multiplied by 100.
For each run, a no template control (NTC) and serial percentage control (0%, 10 %, 25 %, 50 %, 75and 100%) in triplicand were included.
This analysis provides the levels of overall DNMT activity and is not specific to any particular gene or to any particular DNMT, and data are represented in terms of percentage control.
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"We continue to successfully execute our business model at historically high levels, while improving our gross margin percentage, controlling our expenses and generating significant positive cash flow," Mr. Jacobson said.
All samples and DNA percentage controls were performed in triplicate.
The most preferable annealing temperature would be the one that could differentiate between the serial percentage controls.
Inhibition rates were calculated as percentage controls using the formula: (4) % inhibition = 100 − % reaction, where % reaction = (mean product in sample/mean product in control) × 100.
Results from triplicates (means of counts per minute ± standard deviations) are expressed in percentages (control taken to be 100%).
Data are expressed as percentage of control with control concentrations noted in the figure legends.
Where applicable, the percentage of control and percentage of control SEM for each treatment condition were calculated by dividing the raw means and raw SEM's by the control condition raw mean.
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