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In order to keep the false positive rate low, extra parameters were considered: 1) percentage alignment between query and hit ORFs; 2) percentage conservation between aligned portions of query and hit ORFs; 3) ribosome binding strength (see details further down).
The total number of reads per sample and the percentage alignment are summarized in Additional file 3: Table S1.
However, this EST still has the highest percentage alignment with NCX1 genes in comparison to the other three paralogs from other species.
The specificity of tag identification depends on the length of the tag sequence entered, and the predefined thresholds specified for sequence tag identification including the percentage alignment threshold, gap penalty, match and mismatch score.
P is iteratively extended from the current last node to the node connected with the highest edge weight, provided that it has a specified minimum alignment length l A,min and minimum percentage alignment identity f A,min.
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Furthermore, when a comparison of the relative positions of ancestrally segmentally duplicated regions of the rice genome was made with the relative numbers of percentage alignments with the test databases, the two were found to be co-incident in terms of both positive, significant correlations (Table 3) and heat-maps.
After removing low-quality reads, a total of 150 million high-quality clean reads remained, of which 90.48 92.15% were aligned to the reference genome using TopHat (Table 1).> -wrap-foot> Percentage of alignment = high quality reads aligned to genome/high-quality reads.
Analysis on single pattern structures showed that 2 μm gratings with 2 μm spacing and 2 μm height (2 μm gratings) and 2 μm gratings with hierarchical structure produced cells with the highest gene expression of TH and PITX3, with the longest neurite and highest percentage of alignment.
Table 5 shows the mean pairwise percentage of alignment sites with a gap, calculated separately for comparisons within Drosophila, within Sophophora, and between the two clades.
Additionally, we assessed the percentage of alignment for each library.
The best hit (that with the highest percentage of alignment) from each read was used for a particular genome coverage calculation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com