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After this, each of the resulting 157 single-protein data sets were concatenated into a single large alignment containing 72 taxa with 41,372 unambiguously aligned amino acid positions (the total percent of alignment positions in the "157-protein" data set that are gaps are shown in supplementary table S1, Supplementary Material online).
The percent of alignment columns identified as putative SDPs is 1.2 in the EC Pfam dataset and 1.7 in the experimental dataset.
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Then, the specificity is defined as the percent of yeast alignments that have a significant match in MIPS (P<0.05), and the sensitivity is defined as the percent of MIPS alignments that have significant matches in the resulting alignments.
(B ) Single nucleotide polymorphism (SNP) and insertion deletion polymorphism (INDEL) puzzles presented separately and color coded with a heat map depicting the percent of read alignments contributing to the difference between player and software.
For each of the 3 matches, the number of ambiguous bases (International Union of Pure and Applied Chemistry codes) and the percent aligned (alignment length as percentage of query length) were calculated.
To aid in evaluating the networks, we create quartile plots of alignment percent identity, alignment length, and edge count versus edges binned by associated E-value (see Fig. S7).
There was also a positive effect of percent alignment length on hybridization ratio, although unaffected by %ID.
Given the broad distributions of percent identities, alignment lengths and e-values for alignments of type 1 peptides, it seems reasonable to expect that biases in the databases affect these distributions, further confounding assignments based on alignment alone.
Stitch filters by the percent of the total possible alignment length that is aligned (Fig. 3 c).
The percent sequence identity of alignments was very high among presumptive orthologues, ranging from 84 100% at the more stringent E threshold of E<10-80.
When used for a progressive alignment this considerable enhancement in speed came at a cost of several percent in alignment accuracy, as benchmarked on the Pfam database of aligned protein families [ 15].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com