The expansion screw was activated one quarter of a turn per day (0.25 mm per turn) until the palatal cusps of the maxillary posterior teeth approximated the lingual cusps of the mandibular posterior teeth.
Mixed stage populations of N2, xpa-1, nth-1 and nth1;xpa-1 were reared at 20°C on HT115 DE3 -seeded HT115 DE3 -seeded plates per replicate, 3 replicates per strain) until the nematones had cleared the plates of food.
SDS-PAGE was carried out at a constant current of 15 mA for 30 min per gel and then 30 mA per gel until the bromophenol blue reached the bottom of the gels.
Gels were run for 1 h at 1 W per gel followed by 13 W per gel until the bromophenol blue had migrated to the bottom of the gel.
Electrophoresis was conducted at 10°C for one hour at 5 W per gel and afterwards at 17 W per gel until the dye front reached the end of the gel.
The samples were cooled at a rate of maximum 5 °C per hour to 0 °C and subsequently at 2 °C per hour until the selected temperature was reached.
By January the group will be culled from 55 to 33, three players per position, and they will keep attending, three days per month until the summer when there will almost always be a tournament.
It was done at 0.33 mm four times per day for 4 days and then three times per day until the desired length was obtained.
After this, the crude oil price remained relatively stable within a corridor of about 100 to 120 USD per barrel until the recent sharp fall to about 40 USD per barrel at the end of 2015.
The vertical gels were run at 10 mA per gel for 30 min followed by 25 mA per gel until the bromophenol blue had migrated to the bottom of the gel.
