Sentence examples for per ug of from inspiring English sources

To fragment DNA, we created a master mix of DNase I (Promega), One-Phor-All BiosciencesrshandBiosciences), Acetylatedated BSA (Invitrogen); the amounts added per sample were 4 µl 10X One-Phor-All, 0.14 µl Acetylated BSA, and approximately 0.085 µl of DNase per ug of DNA.

Specific activity per ug of gastric extract was defined as units of activity of 1 ng of EWL on M. luteus, at pH 5.5.

5 U of RNase-free DNase per 5 ug of RNA was incubated at 37°C for 30 min, followed by 10 min heat inactivation at 65°C.

The cost per 1 ug of plasmid DNA prepared using glass syringe filters is less than a third of that for DNA prepared using commercial kits (Table. 1).

The amount of phosphoprotein in ng per 20 ug of total protein lysate was then determined by comparing the measured absorbance of the sample to the standard curve.

The three cell lines were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and 250 ug of G418 per ml.

Equimolarly pooled BAC DNA samples were sent to Applied Biosystems as per their requirements: 49.7 ug of B6 BACs and 69.3 ug of D2 BACs.

Experiments were repeated 3 times and the absolute quantitative method was used to determine copy numbers of TLR4 and 18S rRNA per ug input reverse-transcribed total RNA.

40 ug of protein per sample was separated using sodium dodecyl sulfate-polyacrylamide electrophoresis and transferred onto Nitroplus membranes (MSI, Flanders Ma) using a semi-dry transfer method.

The assay of the chitin content was carried out according to the method described by Arakane et al (2005).[18] Early day one of the fifth instar larvae were used for the injection of 500 ug of trehalose per individual (the fifith instar larva has about 250 ug trehalose in the haemolymph).

The CHL-resistant rickettsiae were designated as R. montanensis pMODompBCAT\GFPuv line 700 (Rmontandnsis700) and were serially passed by inoculation of 0.1 ml of infected culture onto new ISE6 cell cultures maintained with 1 ug of CHL per ml and are currently at the 29th passage.