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This was then used to calculate an initial rate of chloride transport per transporter molecule, taking account of the size of the vesicle.
Assuming the rate of transport per transporter of 10 3 molecules s and our cell's surface area this requires about 10 4 transporters μ m 2. This is about an order of magnitude higher than the number of ATP synthase complexes on the thylakoid membrane in spinach, 700 complexes μ m 2 (Miller and Staehelin, 1979).
However, due to the estimated density (10 to 1000 per µm2) and transporting efficiency (10 to 104 ammonium per second per transporter) [26], it only functions in a very low ammonium level or low pH environment [6].
We observed 2 3 mutations per kilobase in the selected transporter genes, resulting in 3 5 amino acid substitutions per transporter.
The analysis was performed for 56 GUVs from 6 experiments (all with 0.01 % transporter), giving an average initial rate per transporter of 820±260 Cl− s−1.
For instance, SLC35A1-A3 (NSTs) have one documented substrate each, whereas SLC35B2-3 (UAA) both share the same substrate, and SLC35D1 and D2 (TPT) have multiple substrates per transporter [additional file 15: supplementary table S11].
Similar(53)
A PLACE analysis has been applied to these promoter sequences and the 216 identified cis-acting elements have been classified per sugar transporter subfamily, for comparison.
Taken together, an ABC transporter has the ability to utilize much more energy per transport cycle than a secondary active transporter, allowing the transport of more energy-demanding substrates.
Therefore, we consider hereafter that only one ATP molecule is consumed per transport cycle for a P-gp like transporter.
Our research group earlier demonstrated that cotransporters function as unconventional water transporters carrying 300 500 water molecules along with the transported solutes per transport cycle18,22, rendering the combined solute and water transport near-isotonic.
Cell-type differences in hexose transport can be anticipated because catalytic turnover rate per molecule of transporter (kcat) for the neuronal GLUT3 for glucose is about six times higher than the astrocytic GLUT1 (Simpson et al., 2007).
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