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In the second approach expression was quantified on a "per transcript" basis.
Thus, the "per transcript" approach results in a considerable loss of information.
This circularization is thought to increase translation [ 44] which in this case would be targeted to the Per transcript.
Hence, the phenotype of RBM47 loss depends on the intracellular molecular milieu on a per transcript basis.
Secondly, we observed a significant reduction of total transcription times and dictation time per transcript after the intervention.
Transcription initiation and termination are clearly one-time events per transcript.
There was a significant decrease of the average dictation time per transcript with the increase in seniority.
Among different groups, there was a significant decrease in the average dictation time per transcript with the increase in seniority.
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In an effort to be conservative, DESeq chooses as its estimate of dispersion the maximum of a per-transcript estimate and the functional form Eq. 2 which is fitted to the per-transcript estimates for all transcripts.
Length and per-transcript %GC content were calculated using BEDTools (version 2.16.2 [ 42]), using the same gene models described above.
As for edgeR, the package NBPSeq [ 33] considers per-transcript log likelihood conditioned on the sum of counts across replicates.
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