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Transcript diversity was captured by assembling reads on a per tissue type and per time point basis pooling the biological replicates within each sample/treatment.
The assembled data set consisted of three biological replicates per tissue and/or time points for both G. raimondii and G. arboreum.
(B) Representative [18 F]-FDG input and tissue time-activity curves acquired ex vivo using the protocol described in (A) (y-axis units are counts per second).
The stem tissues from 24-, 48- and 96-hr post-inoculation were collected for qRT-PCR validation with three independent biological replicates per tissue sample per time point.
Tissue count I reflects the total number of present instances per tissue type.
where A Tot(t) is the whole 18F-FDG activity per tissue unit volume at time t (kBq.mL−1), which is corrected for 18 F physical decay (and includes trapped tracer and free tracer), W is the patient's weight (kg), and ID is the injected dose (MBq).
Equation 2 yields the TAC of trapped 18F-FDG per tissue unit volume at time t, i.e. A Trap(t): A Trap t = λ C Trap t = K ∑ i = 1 3 λ C i ( e − λ + k R t − e − α i t ) / α i − λ − k R (3).
AT t) (in kBq mL−1) is defined as the total tracer activity at time t per tissue volume unit that includes both trapped tracer and free tracer in the blood and interstitial volumes.
Five fields per tissue and 11 mice per time point were examined.
SPC refers to the amount of spores produced per unit area of sporulating tissue per time unit (Pariaud et al. 2009b).
Four biological replicates in each of 5 time-points per tissue (skin and spleen), which comprise a total of 40 microarrays (one array per sample), were included in the analyses.
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