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Serial sections of 5 µm thickness were cut using a microtome, at ten sections per slide.
Intensity was analyzed in two regions of the corpus callosum per section at three sections per brain for two animals per time point.
The total number of hemorrhages was tabulated for all ten sections per mouse.
At least six images per section, four sections per sample, and three samples per group were included to obtain the calculation.
Presented as one section per block; four sections per case.
N = 4 mice per group (four sections per mouse).
Data were obtained from six to eight nonoverlapping fields per section and four to six sections per treatment, 3 5 mice per treatment.
Twenty serial sections were cut per tissue, with three to five sections per slide.
Three areas were assessed per section, from each of three sections per cell type and growth-factor treatment.
We performed serial sections to produce 20 unstained slides per tissue, with three to five sections per slide.
In total, 20 fields of vision per kidney section (three to four sections per kidney) at ×200 were observed blindly as a semiquantitive assessment of immunohistochemical staining.
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