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Tacon said such payments could amount to tens or even hundreds of thousands of pounds per supplier.
Where images are sourced from multiple suppliers, the applicable reproduction fee above shall be payable on each image to a maximum of GBP 100.00 per supplier, i.e. if a supplier provides more than one image in a multi-sourced gallery or slideshow then a total fee of GBP 100.00 shall be payable to that supplier.
Total RNA from SAEC was isolated by using RNeasy kit (Qiagen) as per supplier's instructions.
Total RNA was isolated from differentiated SAEC treated with IL-13 with or without AR inhibitor by using RNeasy kit (Qiagen) as per supplier's instructions.
Cell cultures were fed with fresh growth media every 3 4 days, grown to 70% 80% confluence, and then subcultured with Trypsin-EDTA (Lonza), per supplier's protocol.
Standards were solubilized in DMSO and molarity was determined per supplier recommendations.
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The medium was changed every day, per suppliers' protocol.
All cells were maintained and cultured as per suppliers' recommendations.
Primary antibodies used as per suppliers instructions were ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), (Cell Signalling Technology).
The mice were housed under a 12-h light/dark cycle and bred as per the supplier's protocol [14].
Subsequently, they were transfected using GeneJuice Transfection Reagent (Novagen, Madison, WI, USA) as per the supplier's protocol.
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