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For 2D tissue slices, the blood vessels could be stained and then one could count the number of blood vessels in a series of non-overlapping circles to obtain a histogram of the density distribution in units of number of microvessels per unit area, e.g., per square mm.
MAPD-3N with a high pixel density of 15,000 per square mm and area 3×3 mm2 produced by Zecotek were used.
We have measured several important performance parameters including: the output count rate (OCR) in excess of 20 million counts per second per square mm with a minimum loss of counts due to pulse pile-up, an energy resolution of 7 keV full width at half-maximum (FWHM) across the entire dynamic range, and a noise floor about 20 keV.
The vehicle group had an average of 500 TSPO+ cells per square mm, while the etifoxine treated group had 122.
The control group averaged 717 Iba1+ cells per square mm compared to the drug treated group 153.
The ultimate strength (MPa) was determined as the maximum force applied per square mm recorded during the experiment.
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PyMT mice positive for mCAT showed a more than two-fold decrease in the average number of metastatic foci in the lungs, as well as a 10-fold decrease in the metastatic tumor burden, compared with mCAT negative PyMT littermates (0.1 square mm per square cm of lung tissue versus 1.3 square mm per square cm of lung tissue, respectively, P ≤ 0.01) (Table 1).
The measured value per hpf was converted to the value per square millimeter (mm) by using the conversion factor × 0.0625-1 [ 28].
The numbers of positive cells were expressed as the number of positive cells per square millimeter (mm) of epidermis or dermis.
Mitotic rate is determined by the number of mitoses per millimeter squared (mm) in the invasive melanoma component and all sections on the slides should be examined to evaluate for mitoses or "hot spot".
The cells were thawed and seeded in α-minimum essential medium (α-MEM, Gibco, invitrogen, USA), 10% fetal bovine serum (FBS, Gibco, invitrogen, USA), Grown and confluent cells were trypsinized, collected, and plated at 500 cells per square centimeter in 100 mm plates (Corning incorporated, USA).
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