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Log2 ratios were normalised per spotted sub-array by subtraction of the median value of all BAC clones spotted within that sub-array.
On average, we predicted 74 candidates per ancestral strain, 21 candidates per typhus strain, 152 candidates per transitional strain, and 158 candidates per spotted fever strain.
Thus ~1,000 unique constituents would be represented at approximately one per spotted position since the colonies were diluted to contain one colony per well volume.
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The data were normalized using per spot and per chip LOWESS normalization.
Normalisation was performed using a 'Per Spot and Per Chip' intensity dependent (Lowess) normalisation using software defaults (20% smoothing/cutoff 10).
Initial data were preprocessed by employing the enhanced Agilent FE import method, and then per-spot and per-chip normalizations were performed for all arrays.
Raw data was preprocessed via the enhanced Agilent FE import prior to per-spot and per-chip normalizations for each array.
12-14 control gDNA probes were spotted per subarray.
Up to 85 arrays were spotted per batch.
A total of 30 μl extract was spotted per lane.
It charges $5 per spot.
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