A similar procedure was performed for NFH staining and the two measurements divided to form the "Myelination Index" — as a measure of the amount of myelin sheath per axon area.
In this method, the mean leaf primary production corresponds to the mean number of leaves produced per shoot and per year multiplied by sheath and blade biomass.
We also show that activation and reduction of Fyn kinase in oligodendrocytes increases and decreases sheath number per cell, respectively.
This increase in sheath number per cell occurred without any change in oligodendrocyte number or myelin sheath length, indicating that more axonal space was myelinated in Tg mbp ca-Fyn) animals.
To test further whether the generation of new myelin sheaths by individual cells is restricted to this period of only a few hours in the life of the oligodendrocyte, we sought to identify manipulations capable of increasing and decreasing myelin sheath number per cell.
The extent of myelination can be regulated by neural activity (Fields, 2010; Wake et al., 2011; Zatorre et al., 2012) and by social isolation of juvenile mice, which causes a reduction in myelin sheath number per oligodendrocyte and correlates with poor performance in learning and memory tasks (Makinodan et al., 2012).
By analyzing single cell morphology in such animals, we observed a 33% increase in myelin sheath number per oligodendrocyte in Tg mbp ca-Fyn) animals (wild-Tg mbp ca-Fyn 33 cells; Tg(mbp:wt-Fyn), 15 ± 5, 32 cells; Tg(mbp:canimals 20 ± 6, 36 cells, p < 0.01).
Although PVI was successfully achieved in all included patients without the help of a deflectable sheath (not allowed per protocol), we feel that this curve was not always optimal for PVI and that it might have negatively impacted the CF and FTI in a significant number of patients in our study.
g-Ratios were determined by dividing the diameter of each axon by the diameter of the same axon together with its myelin sheath in 1,000 2,000 axons per animal (n = 3 5 per genotype) using ImageJ software.
Fourteen days after inoculation, all treated fascicles were removed from the plants and the needles (three per fascicle) separated from the fascicle sheath.
