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To weigh these proportions in relation to total number of leaves per seedlings, we used GLMMs with distance and plateau as fixed effects and maternal tree as random effect, and with binomial error structures.
Non-parametric Mann–Whitney U-test [ 67] (p < 0.01; 2-tailed hypothesis) was performed to determine if there are significant differences between the atrad21.1, atrad21.3 and atrad21.1 atrad21.3 mutants and the wild type plants Col; differences in hypersensitivity to ionising radiation exposure were estimated via the number of true leaves per seedlings.
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Mean seedling height and number of leaves per seedling followed a similar pattern.
Two flowers per seedling were used, and 7 to 30 seedlings were examined for each test.
There were significant quadratic relationships between sowing depth and seedling height (p=0.034) as well as number of leaves per seedling (p=0.032), both peaking around 5 mm soil depth.
However, mean seedling height and number of leaves per seedling were higher in 5 and 2.5 mm depths than surface and 10 mm depths.
When seedlings at 10 days post sowing (three-leaf stage) were infested with second and third instar nymphs (twelve nymphs per seedling).
At the second-leaf stage, the seedlings were infested with 2nd to 3rd instar BPH nymphs at 10 insects per seedling.
Seedlings at the two- to three-leaf stage were infested with second- or third-instar nymphs at a density of 10 12 nymphs per seedling.
At third-leaf stage, the seedlings were infested with 2nd to 3rd instar BPH nymphs at a rate of 20 insects per seedling.
Total larval weight per seedling decreased significantly from 0.060 g to 0.012 g when the soil pH increased from 5.1 to 5.7.
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