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The number of wilted plants per seedling plate was recorded at daily basis after 17th day post transplantation (no symptoms were observed before day 17).
Total larval weight per seedling decreased significantly from 0.060 g to 0.012 g when the soil pH increased from 5.1 to 5.7.
Note that the plants from individual single seedling plates were not considered as independent replicates, but instead the number of wilted plants per seedling plate was used as a disease index.
Although there were differences in seedling heights, those grown at 25 mg N L−1 were taller than those at 100, there were few differences in water use per seedling.
Seedlings at the two- to three-leaf stage were infested with second- or third-instar nymphs at a density of 10 12 nymphs per seedling.
At the second-leaf stage, the seedlings were infested with 2nd to 3rd instar BPH nymphs at 10 insects per seedling.
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First, C. camphora was replaced by a surrogate arbuscular mycorrhizal species, Celtis sinensis Pers., for seedling treatment; hence this species occurred only as a seedling species, not as a resident forest species.
After 1 week, we removed the transparent hoods and surplus plants to leave one seedling per well.
To weigh these proportions in relation to total number of leaves per seedlings, we used GLMMs with distance and plateau as fixed effects and maternal tree as random effect, and with binomial error structures.
Oat seedling density was reduced to two seedlings per pot.
We collected seedling leaves from 30 to 70 seedlings per inbred stock when the seedlings were ∼12 cm in height.
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