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All reactions were run in triplicate and for two different vials per sampling date 6 qPCR reactions per sampling date.
A triplicate set of POCIS samplers was deployed in each cage per sampling location for a 2-4 week period.
Eight hundred grams (800 g) of ready-to-eat lettuce and cabbage each per sampling site were taken from the farms and market centres (Table 1).
The qrt-PCR experiment was carried out using the crude extract derived from 20, 8, 2, and 0.2 cells per sampling day.
Next, the averages of these 500 coefficients per sampling point purely based on chance were computed.
One to 8 samples were taken per sampling day and abattoir.
The trapping effort per sampling event was normally 150 trap-nights per 1-ha plot.
The average total trapping effort per sampling occasion was ≈ 8.440 trap nights.
The number of haplotypes per sampling site ranged from one to five, and decreased from south to north.
Two individuals per morphospecies per sampling event were included in the genetic analyses, for a total of 90 specimens.
Thus the effective LOD per sampling event is estimated to be 38 fmol for LE and 494 fmol for AT.
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