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Experiments were run in a completely randomized design with triplicates (3 treatments × 3 replicates × 10 subjects per replication) for 6 months.
The experiment involved four replicates following a completely randomized design with 30 fish per replication.
SEM analysis was run using three biological replicates with at least four technical repetitions of developing grains per replication of the mounted specimens.
We group the results per replication mechanism.
The experimental unit consisted of one plant per replication.
Ten replications are simulated with 1 year (4 quarters) per replication.
The sample number per treatment were 30 plants (10 plants per replication), then we used total 120 plants.
Phenotypes were evaluated on field grown plants in Stuttgart, Arkansas over 2 years (two replications, three plants per replication) as illustrated in Fig. 3.
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Calculating per-replication event mutation rates from observed mutant frequencies is not possible, or even meaningful, without knowledge of the replication mode.
By inferring the mode of replication, we have been able to link estimates of per-replication event mutation rates to published mutant frequencies.
Thus, estimates of poliovirus per-replication event mutation rates can vary over 10-fold depending on the assumed replication mode (Drake, 1993; Sanjuan et al., 2010).
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