Sentence examples for per reactions from inspiring English sources

cDNA amplified using TaqMan Universal PCR Master Mix with 400 nM primers and 200 nM fluorogenic probe in a total reaction volume of 25 µl: In general, cDNA generated from 50 ng input total RNA was used per reactions, with the exception of 18 s detection, where cDNA derived from 50 pg input total RNA was used.

A total volume of 20 μl per reactions was incubated in 96-well MicroAmp plates (Applied Biosystems) for 10 min 95°C followed by 40 cycles of 15 sec.

Calculated oocysts per reactions were based on starting concentration of oocysts per mL of stool, amount of stool used in each extraction, elution volume, and volume of DNA elution used per reaction according to details described in the Materials and Methods sections.

The assay detects down to five gene copies per reaction.

The sensitivity limit was estimated between 0.055 and 0.55 pg of DNA per reaction, equivalent to 1 10 organisms per reaction.

Cloning includes a base fee, while the others are priced "per reaction".

Estimated detection limits in reagent-grade water were five genome equivalent copies (GEC) per reaction and two to four rotavirus particles per reaction.

The limit of detection was <180 colony forming units per reaction of toxigenic C. difficile.

The optimum copy number of IAC was determined to be 500 copies per reaction.

The assay had a detection limit of four plasmid HPV DNA copies per reaction.

The newly designed assay has a limit of detection of 10 copies per reaction.