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Such a large number of ESWs has not often been observed in the diffusion region as per previously published works.
As per previously published aging protocols (Fig. 4a, b) [11], this procedure was repeated, this time with yellow rather than brown food colouring, thereby subjecting the aligners to a total of two 14-day aging cycles.
After the last puff, QCMs were left up to an additional 10 min to reach plateau phase, where recorded mass ceased to increase further, as per previously published dosimetry protocols on both machines [2, 3].
Of 65 initially uninfected deer that we sampled in at least two consecutive years (n = 77 deer×years of presumed susceptibility), 19 became infected (estimated incidence = 0.23 new infections per previously uninfected deer per year; 95% binomial CI 0.15 0.34 new infections per previously uninfected deer per year).
30 Reproducibility was determined per previously described guidelines.
The mRNA extraction was completed as per previously described protocols.
as per previously developed antibody-coupling protocols (Schwenk et al, 2008).
Turbidity evaluation of the formulated emulsions was performed as per previously reported method [ 18].
Tumour samples were fixed in formalin and embedded in paraffin as per previously established protocols.
Subjects underwent quantitative sensory testing as per previously established protocols involving thermal stimuli [ 29, 30, 45, 46].
As per previously established endpoints [ 21], mice were sacrificed at approximately 12 weeks post-injection and assessed for metastatic burden.
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