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The absolute level of bioluminescence depended from the respective number of seeds per plate, size, and the coelenterazine uptake of the plants.
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Cells were seeded into drug-prepared microplates at varying density per well, depending on cell type and plate size.
Some of these are technical regulations like emissions standards and even license plate size.
Decide on the best plate size.
Even plate sizes have increased, with dinner plates going from 10 inches to 12 inches over the past three decades.
Use smaller plate sizes.
To account for variability between plates, the colony sizes were normalized using the median colony size per plate.
We used the logit function with a binomial distribution [ 29] to describe the proportion of dauer larvae (p) and arrested L4 non-dauer larvae (q) among lines, ascarosides/pheromone and food treatments, and sample size (n) per plate.
Briefly, the BACs were pooled from 42 384-well plates (i.e. 384 BACs with a 138 kb average insert size which per plate covers ~53 Mb (23%) of the 230 Mb), in a 3-D fashion into row, column and splitbox pools resulting in 22 pools per plate (924 pools in total).
All adult females up to a sample size of 200 per plate were screened.
There is a dinner plate-sized wound.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com