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However, there is always a set of genes that is found to be "increased" when analyzed on a "per one cell" basis that are declared to be "decreased" by global type normalization, or vice versa.
Dependence of the integrated luminosity per one cell on the vertical distance between the cell holder and the suspension surface for two cases: open and confined air.
Figure 2 Relationship between the integrated luminosity per one cell and vertical distance from the NP source.
The MathWorks Matlab R2009b and Simulink software (Torrance, CA, USA) were used for image analyses in order to quantify luminosity per one cell.
Figure 2 illustrates the relationship between the integrated luminosity per one cell and vertical distance from the cell holder to the suspension surface (see relationship between the integrated luminosity per one cell and horizontal distance from the NP source in Figure S1 in Additional file 1).
Isolated (a) and non-isolated (b) from the air environment of a cell culture, (c) labeled with the fluorescent NPs. Figure 5 Dependence of the integrated luminosity per one cell.
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After an hour, the concentration of bacteria dropped from 10 million cells per liter to just one cell per 10,000 liters.
Five hundred cells per well (or down to one cell per well for limiting dilution) were plated in complete neurobasal medium, and sphere formation was measured after 10 days.
The standard curve, which ranged from 5 to 2500 fmol per one million cells (fmol/106 cells), was fitted to a quadratic regression model weighted by 1/concentration.
We're simulating one cell per minute, but they're supposed to operate at a microsecond.
Using only one cell per well, it was extremely difficult to obtain colonies.
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