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All experiments with platelets were performed with cell concentrations of 200 × 106 cells per ml and all experiments with PBMCs with 5 × 106 cells per ml.
K562 cultures were split at 2.5 × 105 cells per ml 1 day before transfection and were at ∼5 × 105 cells per ml when collected for transfection.
Metadata for station 131 is: TΔ. 2.31 °C, prokaryote abundance of 2.8E4 cells per ml and virus-like particle abundance of 1.3E5 VLP per ml.
Vector titers were 2 3 × 106 infectious particles per ml and d120 helper virus titers of 0.4 1.0 × 107 plaque forming units (PFU) per ml.
Metadata for station 134 is: TΔ=2.26 °C, prokaryote abundance of 2.41E4 cells per ml and virus-like particle abundance of 1.14E5 VLP per ml.
Upon homogenization by thorough shaking, the binding capacity is ∼5 mg Sav per ml of suspension.
CK8/18+ and HER2+ cells were counted and plotted per ml of blood.
One ng per ml anti-rabbit IgG-HRP (Santa Cruz Biotechnology) was added to each sample.
T cells were resuspended at 1 × 106 viable cells per ml in cRPMI-10 medium.
Viruses were titered using quantitative PCR to approximately 1013 vector genome copies per ml.
Visible plaques were counted to determine viral titers as plaque forming units per ml (PFU/ml).
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