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The calculated number of specific transcripts was normalised to the housekeeping genes cyclophilin B and hypoxanthine guanine phosphoribosyltransferase, and expressed as number of copies per microlitre of input cDNA.
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When the CD4 count falls to about 200 cells per microlitre of blood (in an uninfected adult it is typically about 1,000 cells per microlitre), patients begin to experience opportunistic infections i.e., infections that arise only in individuals with a defective immune system.
In the past, delayed antiretroviral therapy was the preferred approach, with treatment initiated once CD4 levels had fallen to 200 cells per microlitre of blood, which generally coincides with the establishment of symptomatic disease.
In humans, a regulated steady platelet supply and clearance maintains numbers of 150,000 400,000 platelets per microlitre of blood.
hRSV load is expressed as copies of N gene molecule per microlitre of sample (copies/µl).
*CD4 and CD8 are per microlitre of plasma, viremia is per millilitre of plasma.
This gives an average detection threshold of 16 gametocytes per microlitre of blood.
For qPCR analysis, extracts were diluted to the equivalent 25 μL plasma per microlitre of extract.
Extracts were analysed undiluted by qPCR (equivalent to 20 μL plasma per microlitre of extract).
Their CD4 count at initiation was 400 ± 150 per microlitre of plasma.
Substantial transmission was seen from hosts with <1 gametocyte per microlitre of blood.
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