Exact(18)
The PCR products were extracted using the QIAquick PCR Purification Kit (Qiagen) as per manufactures' instructions.
The reaction was performed on the Biotage PSQ HS96 instrument, as per manufactures instructions.
TUNEL staining was performed as per manufactures protocol.
DNA was purified using DNA purification kit from Qiagen as per manufactures protocol.
mRNA was reverse transcribed using Taqman Reverse Transcriptase Reagents (Applied Biosystems, Branchburg, NJ, USA) as per manufactures instructions.
Caspase 3 activity was measured using the Caspase 3 assay kit from Abcam (ab39383) as per manufactures instructions.
Similar(42)
First strand cDNA synthesis was carried out using Superscript III reverse transcriptase (Invitrogen) and 3 ug random hexamer primers (Illumina) per sample as per the manufactures' instructions.
First strand cDNA synthesis was carried out using Superscript III reverse transcriptase (Invitrogen) and 3 μg random hexamer primers (Illumina) per sample as per the manufactures' instructions.
SP-A was labeled with Alexa Fluor 488 (Invitrogen, Carlsbad, CA) per the manufactures instructions.
Genomic DNA was isolated using Qiagen DNAeasy Tissue Kit as per the manufactures instructions.
The cells were seeded to obtain 80 90% confluence prior to transfection and different doses of DNA were transfected using Lipofectamine 2000 (Invitrogen) as per the manufactures protocol.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com