Exact(29)
The luciferase activity was determined as per manufacture's instructions.
DNA was extracted with the QIAamp DNA mini kit (Qiagen GmbH, USA) as per manufacture's recommendation.
The cytochrome C oxidase (Complex IV) activity was determined by using cytochrome C oxidase kit (Sigma, USA) as per manufacture's instructions.
All curves reached a similar plateau height and, as per manufacture's recommendations, the mean cycle number at which fluorescence exceeded background (referred to as the crossing point or cycle threshold) was <30 with a range of less than 7 across all samples.
Single or multiple doses per manufacture?
An ALDH kit (Stem Cell Technologies) was used to stain ALDH population as per manufacture instructions.
Similar(31)
The reaction was performed on the Biotage PSQ HS96 instrument, as per manufactures instructions.
The PCR products were extracted using the QIAquick PCR Purification Kit (Qiagen) as per manufactures' instructions.
TUNEL staining was performed as per manufactures protocol.
DNA was purified using DNA purification kit from Qiagen as per manufactures protocol.
GFP containing vector was transient transfections were done using Gene Porter 2 (Genlantis, Interscience, San Diego, ON, USA) as per manufactures instructions.
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