Exact(3)
Finally, matches were counted and RPKM computed per cluster and per libraries.
These libraries were sequenced to a depth ≥100,000 tags (total number of tags per libraries, see Fig. 1b).
16 to 22 million reads per libraries were obtained and aligned against the Arabidopsis cDNA representative_gene_model (TAIR 10) using BWA (Li and Durbin, 2009) and Samtools (Li et al., 2009).
Similar(57)
Our direct cloning approach served two basic purposes: discovering new miRNA candidates and obtaining rough frequencies in a per library manner.
The expression values are shown in tags per million (TPM) calculated on a per-library total expression.
The average per-library coverage was 1,794, with a minimum coverage > 100 in 99.7% of the genome.
The rest of the $3 million to $5 million per library comes from the city.
High quality of the generated libraries was verified by sequencing five clones per library (GATC Biotech, Cologne, Germany).
Over 80% clean tags per library were mapped to the reference database, showing the DGE data was reliable and sufficient for subsequent bioinformatics analysis of gene expression.
Over 100,000 transformants per library were generated with this method.
However, the number of 5'SOLiD tags identified per library sample is one hundred-fold greater than the number of 5'SAGE tags detected per library sample.
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