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Insulin content per isolated islet was measured by ELISA [12].
We established a new method to estimate islet volume via the total cell number per isolated islet in rats.
The serine and glycine peaks per isolated, and the peak areas were normalized by leaf fresh weight.
Five recombinant bacterial colonies (white) per isolated band were picked from the plate containing ampicillin and X-gal as selective media and cultured overnight at 37°C.
Consequently, the mean number of fibres per isolated anatomical structure differs substantially among the anal sphincter complex, perineal body, the puboperineal muscle and the internal obturator muscle [ 8].
The upper (paralog 2) and the lower (paralog 1) bands were independently purified in each individual and cloned using the TOPO-TA cloning kit (Invitrogen Life Technologies, Carlsbad, CA, USA) as described above, with the exception that twenty recombinant bacterial colonies (white) per isolated band were picked from the plate.
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We estimate that the per isolate cost to perform MLST using HRM is 20 30% that of sequencing.
On a per isolate basis, bap carriage was not associated with biofilm formation, as determined by CV uptake (p = 1.00).
For analyses of covariance (ancova), we calculated means of SPC and LP20 per isolate (n = 6 means in experiment 1) and per isolate × host variety combination (n = 41 means in experiment 2, see Table 1).
An average of 11.3 × 106 monocytes were isolated per sample.
Approximately 0.5-1.5 0.5-1.5 were isolated per calf lung.
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