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In single-author papers, weA forms are used sparingly, with some striking individual exceptions.
There are some individual exceptions.
Environmental chemicals (see Supplemental Material, Figure S1A C), generally show a slight decrease in recent cycles in the average amount per individual with notable exceptions (e.g., enterodiol; see Supplemental Material, Figure S1A).
The number of CNVs per individual ranged from 35 to 65, with the exception of one individual who is described more fully later (Table 2).
PCR amplicons were cloned into the pCR II TOPO vector (Invitrogen) with six clones picked per individual and sequenced for all genes with the exception of Arginine KInase and Wingless, where the PCR amplicons were directly sequenced.
The exceptions were trnT- trnF which required two PCR reactions per individual using trnT-a/ trnL-d and trnL-c/ trnF-f [ 59].
RNAi clones were re-transformed into HT115 DE3) and RNAi was conducted as above, with the exception that single L4 hermaphrodites (n = 12) were placed on individual plates to determine brood size per individual animal.
(2) One Identifier per Individual.
Limit of 2 tickets per individual.
(An annual pool badge is $20 per individual resident).
(b), Percentage of heterozygous genotype calls per individual.
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