At 25N, a flexible menu of options starts at $250 per month, which is on par with individual area office rentals.
This difference was significant both by analysis of means per timepoint and by analysis of individual areas under the curve.
Home range sizes ranged from 125 to 2551 ha (median: 403 ha), with a median number of 2 core areas (range: 1 5 core areas per individual per year).
Next, we counted the number of sarcomeric disarray, irregular Z-line, and filament disorganization occurrences in a 590-µm2 590-µm2area per individual and calculated the percentage of these abnormuscleructures (number of arearmal structures/number of observed).
All the leaves were immediately set in distilled water for a minimum of 12 h to reach full hydration [ 74] and total leaf area per individual (TLA, m) was determined then with a planimeter (Light box, Gatehouse, Scientific Instruments LTD, Norfolk, UK).
In order to independently manipulate density (5 treatments: 80 to 248 cm2 surface area per individual; Figure 2) and group size (3 treatments: 2, 4, and 8 individuals per container; Figure 2) we built 15 customized experimental containers using 3 mm acrylic glass and Acrifix® 120 (Seven Hills, Australia) acrylic glue.
Because movements of C. sandrana are restricted to the benthic substrate, i.e. a 2-dimensional space with equal usage of vertical and horizontal planes (personal observation), we only varied surface area per individual (see below), but not the water volume (612 ml per individual across all treatments) by placing each experimental container into a larger standardized water tank.
The average number of immunoreactive cells per area and per individual was divided by the number of sections containing that area and compared between treatment groups.
Expected values of species richness were obtained using rarefaction, which estimates species richness on a per-individual, rather than a per-area, basis [ 2, 36, 37].
Modeling healing time is a complex statistical problem, with random effects due to multiple burn areas per individual, and censoring caused by patients missing hospital visits and undergoing surgery.
Because mate availability can be both a function of social group size (the total number of interacting individuals) and density (the number of individuals per area) we applied an experimental approach that, in theory, is capable of differentiating between both effects.
