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Following a bioinformatic analysis of EST data using a semiautomated pipeline, 1885 representative ESTs (rESTs) were selected, to which oligonucleotides (three per EST) were designed and spotted on to a microarray.
Two or three 60-mer probes were designed per EST, and all probes were printed on the array to account for technical replicates.
The number of eQTL per EST varied from 1 to 8 with a mean of 3.
To merge the BC1 and 10-5 maps, which contained one SNP per EST, to the QTL-BASE2 consensus map, which contained one to three SNPs per EST, it was necessary to select the most informative SNP within ESTs.
The results for each species, regarding the percentage of SSRs found per EST database size are shown on Table 2.
The read count per EST was ranged from 42 to 182,192 with the median of 174.
Similar(32)
CD74 is the most abundant one among the surface markers, which contained 78 ESTs (9,834 transcripts per million ESTs), comparing with 11,533 transcripts per million ESTs in the human lymph node.
The number of alleles amplified per EST-SSR ranged from 2 to 8, with an average of 2.9 (Fig. 2, Table 4).
However, the most frequent number of alleles amplified per EST-SSRs was 2, suggesting that these markers were probably generated by insertion deletion polymorphisms, as previously observed in soybean.
The numbers of SSRs per ESTs in the two root libraries were similar, with 5.4% for the HP library and 4.8% for the LP library.
Patient travel costs Not Reported Did not perform subgroup analysis AUD$, 1999 CR program cost: $21.57 per session per patient Est.
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