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The effect of matrix co-extractives was performed by assessing ion suppression or enhancement effects of signals from chromatograms of matrix matched standard solutions compared to spiked extracts at the same concentration levels as per DG SANCO guidelines for LC-MS/MS analysis (SANCO/12571/2013).
The data was presented as number per mm in the cortex or per DG region.
The total number of labeled GFP+ cells per DG was then estimated by multiplying the number by 6.
When observing the LD matrices per DG (data not shown), significant p-values were preferentially located close to the diagonal, and hence, between linked markers.
This potential source of false negative error can be reduced by greater depth of overall sequencing and by setting criteria that require more reads per DG marker for genotype assignment within heterozygous haplotypes.
Second, accurate assignment of HET genotypes requires more sequence reads per DG marker in order to be certain sufficient reads from both alleles have been acquired, if present, and an accurate ratio of allele sequences has been obtained.
Similar(52)
a P values were calculated by using Mann-Whitney test; b P values were calculated by using the chi-squared test; cmm per 1 hour; dG per liter (normal range is 1.0 to 4.8).
Absolute amounts of FLys were always greater (FLys per 10 lysine vs HM-dG per 10 dG).
8-oxo-dG level was expressed as the molar ratio of 8-oxo-dG per 10 dG.
In unexposed cells, we detected levels of 8-oxo-dG about 0.2 per 10 dG.
The 8-OHdG level was represented as the number of 8-OHdG molecules per 10 dG.
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